![]() In order to acquire images on faster timescales, laser scanning confocal microscopes must be re-engineered to incorporate advanced scanning scenarios that enable the beam to be raster-scanned across the specimen at higher speeds. This translates to a scan rate ranging from 500 milliseconds to 2 seconds, depending upon the image dimensions. Unfortunately, traditional laser scanning confocal microscopes are limited in acquisition speed by the galvanometer mirrors, which are driven with a linear saw-tooth control signal at the rate of several microseconds per pixel. ![]() ![]() The growing application of fluorescent proteins in live-cell imaging, however, now requires microscope imaging speeds on the millisecond timescale in order to unravel the intricate dynamics that occur in many biological processes. ![]() Laser scanning confocal microscopy has proven to be a useful tool for examining fixed and stained cells, tissues, and even whole organisms at high contrast by the elimination of light originating in regions removed from the focal plane. ![]()
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